82720-4  Natural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel: -: Pt: Bld MC: -:  

PANEL HIERARCHY  (view this panel in the LForms viewer)

  LOINC#   LOINC Name R/O/C  Cardinality  Ex. UCUM Units 
  82720-4   Natural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel: -: Pt: Bld MC: -:    
       82724-6   Natural killer cell cytotoxicity.3.13:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.3.13:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.3.13:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  
       82725-3   Natural killer cell cytotoxicity.6.25:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.6.25:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.6.25:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  
       82726-1   Natural killer cell cytotoxicity.12.5:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.12.5:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.12.5:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  
       82727-9   Natural killer cell cytotoxicity.25:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.25:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.25:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  
       82728-7   Natural killer cell cytotoxicity.50:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.50:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.50:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  
       82729-5   Natural killer cell cytotoxicity.100:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.100:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.100:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  
 

NAME
  Fully-Specified Name: 
Component   Property   Time   System   Scale   Method
Natural killer cell cytotoxicity panel  Pt  Bld MC 
  Long Common Name:  Natural killer cell cytotoxicity panel - Blood mononuclear cells
  Shortname:  NK cytotox Pnl Bld MC

BASIC ATTRIBUTES
  Class/Type: PANEL.HEM/BC/Lab
  Panel Type: Panel
  First Released in Version: 2.58
  Last Updated in Version: 2.58
  Order vs. Obs.: Order
  Status: Active

PARTS

Part Type    Part No.  Part Name   
Component   LP231607-5  Natural killer cell cytotoxicity panel 
Property   LP6769-6 
Time   LP6960-1  Pt   [Point in time (spot)] 
System   LP193850-7  Bld MC   [Blood mononuclear cells] 
Scale   LP7747-1 
Fragments for synonyms   LP89546-3  Natural killer 
Fragments for synonyms   LP21059-8  Panel 

RELATED NAMES
  Blood PANEL.HEMATOLOGY& CELL COUNTS Point in time
  HEMATOLOGY/CELL COUNTS Panl Random
  NK PBMC WB
  NK cytotox Pnl Peripheral blood mononuclear cells Whole blood
  Pan Pnl  

CHANGE HISTORY
  Change Type: ADD

INTERNAL FIELDS
  Long Common Name: Natural killer cell cytotoxicity panel - Blood mononuclear cells
  Shortname: NK cytotox Pnl Bld MC
  Fully Specified Name: Natural killer cell cytotoxicity panel: -: Pt: Bld MC: -:
     
  Component Word Count: 0
  ID: 87053
  # of Panel Elements: 7
  Status (Raw): ACTIVE



Selected information about each LOINC that is part of this panel



82724-6   Natural killer cell cytotoxicity.3.13:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.3.13:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.3.13:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  

NAME
  Fully-Specified Name: 
Component   Property   Time   System   Scale   Method
Natural killer cell cytotoxicity.3.13:1 effector to target cell ratio/Positive control  RelACnc  Pt  Bld MC  Qn 
  Long Common Name:  Natural killer cell cytotoxicity.3.13:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname:  NK cytotox.3.13:1 E:T/Pos cntrl Bld MC

TERM DEFINITION/DESCRIPTION(S)
  The natural killer (NK) cytotoxicity profile assesses the functionality of natural killer cells and is used to evaluate patients with various conditions including recurrent, severe herpes viral infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or know monogenic defects. The NK cytotoxicity profile is also used for the evaluation of immune reconstitution post hematopoietic cell transplantation and post immunomodulatory therapy. [http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+interpretive/28562] Peripheral blood mononuclear cells (MC are used as the specimen for NK cytotoxicity assays because the cytotoxicity of the MC population is predominantly due to NK cells. In the cytotoxicity assay, samples of the patient's mononuclear cells (called 'effector cells' or E cells') are incubated with labeled target cells ('T cells') in a series of predetermined ratios, for example, 100:1 E to T, 50:1 E to T, 25:1 E to T. Cytotoxicity is measured as the amount of label released when the target cell is destroyed, and the percent cytotoxicity for a given E:T ratio is the ratio of label released when all of the target cells are destroyed using a cell lysis detergent as an experimental control. Several features of the cytotoxicity profile are important, including the maximum cytotoxicity, the relative difference between the maximum and the minimum, and the change in percent cytotoxicity between sequential dilutions. Different patterns of these measures may be associated with different disease processes. Cytotoxicity may also be reported in terms of lytic units (LOINC: 34439-0), meaning the number of E cells needed to lyse a certain number of target cells.
 
 

PART DEFINITION/DESCRIPTION(S)
  Part: Natural killer cell cytotoxicity.3.13:1 effector to target cell ratio
  Natural killer cells are lymphocytes that identify and lyse tumor cells and cells infected with microbes. The ability of NK cells to recognize cells as foreign is dependent upon inhibitory and activating receptors on the target cells. The inhibitory receptors distinguish between normal and tumor or infected cells by the presence or absence of the major histocompatibility complex class I. The activating receptors are NKp46, NKp30, NKp44 and NKG2D. (http://emboj.embopress.org/content/23/2/255.abstract) In addition to their cytolytic function in the innate immune system, NK cells also function to regulate reciprocal interaction with dendritic cells, macrophages, T cells and endothelial cells and thereby affect immune response. [PMID: 18425107] Patients that experience recurrent severe herpes infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or known monogenic defects or patients who have received immune reconstitution post hematopoietic cell transplantation may lack the normal number or functionality of NK cells. The level of NK cell functionality is determined using titrations of effector cells (that are assumed to be NK cells) that are incubated with the target cells. The level of chromium released by the target cells is then divided by the corrected level of chromium measured in the positive control. The result is reported as a percent.( http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/28562)
 
 

BASIC ATTRIBUTES
  Class/Type: HEM/BC/Lab
  First Released in Version: 2.58
  Last Updated in Version: 2.58
  Order vs. Obs.: Observation
  Status: Active

FORMULA -- READABLE
Natural killer cytotoxic activity = (Cytotoxic activity at X dilution - spontaneous activity)/Maximum activity

MEMBER OF THESE PANELS
  82720-4   Natural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel: -: Pt: Bld MC: -:

PARTS

Part Type    Part No.  Part Name   
Component   LP230187-9  Natural killer cell cytotoxicity.3.13:1 effector to target cell ratio 
     Divisors   LP204182-2  Positive control 
Property   LP35077-4  RelACnc   [Relative Arbitrary Concentration] 
Time   LP6960-1  Pt   [Point in time (spot)] 
System   LP193850-7  Bld MC   [Blood mononuclear cells] 
Scale   LP7753-9  Qn 
Fragments for synonyms   LP89546-3  Natural killer 
Fragments for synonyms   LP21265-1 
Fragments for synonyms   LP21065-5  Positive 

RELATED NAMES
  Blood Point in time Random
  HEMATOLOGY/CELL COUNTS Pos RlACnc
  i Pos cntrl WB
  NK QNT Whole blood
  NK cytotox.3.13:1 E:T Quan  
  PBMC Quant  
  Peripheral blood mononuclear cells Quantitative  

EXAMPLE UNITS
  Unit  Source Type
  EXAMPLE UCUM UNITS 

UNITS (INTERNAL DETAILS)
  Source Type:  EXAMPLE UCUM UNITS 
  Unit: 

CHANGE HISTORY
  Change Type: ADD

INTERNAL FIELDS
  Long Common Name: Natural killer cell cytotoxicity.3.13:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname: NK cytotox.3.13:1 E:T/Pos cntrl Bld MC
  Fully Specified Name: Natural killer cell cytotoxicity.3.13:1 effector to target cell ratio/Positive control: RelACnc: Pt: Bld MC: Qn:
     
  Component Word Count: 0
  ID: 87057
  Status (Raw): ACTIVE


82725-3   Natural killer cell cytotoxicity.6.25:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.6.25:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.6.25:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  

NAME
  Fully-Specified Name: 
Component   Property   Time   System   Scale   Method
Natural killer cell cytotoxicity.6.25:1 effector to target cell ratio/Positive control  RelACnc  Pt  Bld MC  Qn 
  Long Common Name:  Natural killer cell cytotoxicity.6.25:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname:  NK cytotox.6.25:1 E:T/Pos cntrl Bld MC

TERM DEFINITION/DESCRIPTION(S)
  The natural killer (NK) cytotoxicity profile assesses the functionality of natural killer cells and is used to evaluate patients with various conditions including recurrent, severe herpes viral infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or know monogenic defects. The NK cytotoxicity profile is also used for the evaluation of immune reconstitution post hematopoietic cell transplantation and post immunomodulatory therapy. [http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+interpretive/28562] Peripheral blood mononuclear cells (MC are used as the specimen for NK cytotoxicity assays because the cytotoxicity of the MC population is predominantly due to NK cells. In the cytotoxicity assay, samples of the patient's mononuclear cells (called 'effector cells' or E cells') are incubated with labeled target cells ('T cells') in a series of predetermined ratios, for example, 100:1 E to T, 50:1 E to T, 25:1 E to T. Cytotoxicity is measured as the amount of label released when the target cell is destroyed, and the percent cytotoxicity for a given E:T ratio is the ratio of label released when all of the target cells are destroyed using a cell lysis detergent as an experimental control. Several features of the cytotoxicity profile are important, including the maximum cytotoxicity, the relative difference between the maximum and the minimum, and the change in percent cytotoxicity between sequential dilutions. Different patterns of these measures may be associated with different disease processes. Cytotoxicity may also be reported in terms of lytic units (LOINC: 34439-0), meaning the number of E cells needed to lyse a certain number of target cells.
 
 

PART DEFINITION/DESCRIPTION(S)
  Part: Natural killer cell cytotoxicity.6.25:1 effector to target cell ratio
  Natural killer cells are lymphocytes that identify and lyse tumor cells and cells infected with microbes. The ability of NK cells to recognize cells as foreign is dependent upon inhibitory and activating receptors on the target cells. The inhibitory receptors distinguish between normal and tumor or infected cells by the presence or absence of the major histocompatibility complex class I. The activating receptors are NKp46, NKp30, NKp44 and NKG2D. (http://emboj.embopress.org/content/23/2/255.abstract) In addition to their cytolytic function in the innate immune system, NK cells also function to regulate reciprocal interaction with dendritic cells, macrophages, T cells and endothelial cells and thereby affect immune response. [PMID: 18425107] Patients that experience recurrent severe herpes infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or known monogenic defects or patients who have received immune reconstitution post hematopoietic cell transplantation may lack the normal number or functionality of NK cells. The level of NK cell functionality is determined using titrations of effector cells (that are assumed to be NK cells) that are incubated with the target cells. The level of chromium released by the target cells is then divided by the corrected level of chromium measured in the positive control. The result is reported as a percent.( http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/28562)
 
 

BASIC ATTRIBUTES
  Class/Type: HEM/BC/Lab
  First Released in Version: 2.58
  Last Updated in Version: 2.58
  Order vs. Obs.: Observation
  Status: Active

MEMBER OF THESE PANELS
  82720-4   Natural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel: -: Pt: Bld MC: -:

PARTS

Part Type    Part No.  Part Name   
Component   LP230188-7  Natural killer cell cytotoxicity.6.25:1 effector to target cell ratio 
     Divisors   LP204182-2  Positive control 
Property   LP35077-4  RelACnc   [Relative Arbitrary Concentration] 
Time   LP6960-1  Pt   [Point in time (spot)] 
System   LP193850-7  Bld MC   [Blood mononuclear cells] 
Scale   LP7753-9  Qn 
Fragments for synonyms   LP89546-3  Natural killer 
Fragments for synonyms   LP21265-1 
Fragments for synonyms   LP21065-5  Positive 

RELATED NAMES
  Blood Point in time Random
  HEMATOLOGY/CELL COUNTS Pos RlACnc
  i Pos cntrl WB
  NK QNT Whole blood
  NK cytotox.6.25:1 E:T Quan  
  PBMC Quant  
  Peripheral blood mononuclear cells Quantitative  

EXAMPLE UNITS
  Unit  Source Type
  EXAMPLE UCUM UNITS 

UNITS (INTERNAL DETAILS)
  Source Type:  EXAMPLE UCUM UNITS 
  Unit: 

CHANGE HISTORY
  Change Type: ADD

INTERNAL FIELDS
  Long Common Name: Natural killer cell cytotoxicity.6.25:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname: NK cytotox.6.25:1 E:T/Pos cntrl Bld MC
  Fully Specified Name: Natural killer cell cytotoxicity.6.25:1 effector to target cell ratio/Positive control: RelACnc: Pt: Bld MC: Qn:
     
  Component Word Count: 0
  ID: 87058
  Status (Raw): ACTIVE


82726-1   Natural killer cell cytotoxicity.12.5:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.12.5:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.12.5:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  

NAME
  Fully-Specified Name: 
Component   Property   Time   System   Scale   Method
Natural killer cell cytotoxicity.12.5:1 effector to target cell ratio/Positive control  RelACnc  Pt  Bld MC  Qn 
  Long Common Name:  Natural killer cell cytotoxicity.12.5:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname:  NK cytotox.12.5:1 E:T/Pos cntrl Bld MC

TERM DEFINITION/DESCRIPTION(S)
  The natural killer (NK) cytotoxicity profile assesses the functionality of natural killer cells and is used to evaluate patients with various conditions including recurrent, severe herpes viral infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or know monogenic defects. The NK cytotoxicity profile is also used for the evaluation of immune reconstitution post hematopoietic cell transplantation and post immunomodulatory therapy. [http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+interpretive/28562] Peripheral blood mononuclear cells (MC are used as the specimen for NK cytotoxicity assays because the cytotoxicity of the MC population is predominantly due to NK cells. In the cytotoxicity assay, samples of the patient's mononuclear cells (called 'effector cells' or E cells') are incubated with labeled target cells ('T cells') in a series of predetermined ratios, for example, 100:1 E to T, 50:1 E to T, 25:1 E to T. Cytotoxicity is measured as the amount of label released when the target cell is destroyed, and the percent cytotoxicity for a given E:T ratio is the ratio of label released when all of the target cells are destroyed using a cell lysis detergent as an experimental control. Several features of the cytotoxicity profile are important, including the maximum cytotoxicity, the relative difference between the maximum and the minimum, and the change in percent cytotoxicity between sequential dilutions. Different patterns of these measures may be associated with different disease processes. Cytotoxicity may also be reported in terms of lytic units (LOINC: 34439-0), meaning the number of E cells needed to lyse a certain number of target cells
 
 

PART DEFINITION/DESCRIPTION(S)
  Part: Natural killer cell cytotoxicity.12.5:1 effector to target cell ratio
  Natural killer cells are lymphocytes that identify and lyse tumor cells and cells infected with microbes. The ability of NK cells to recognize cells as foreign is dependent upon inhibitory and activating receptors on the target cells. The inhibitory receptors distinguish between normal and tumor or infected cells by the presence or absence of the major histocompatibility complex class I. The activating receptors are NKp46, NKp30, NKp44 and NKG2D. (http://emboj.embopress.org/content/23/2/255.abstract) In addition to their cytolytic function in the innate immune system, NK cells also function to regulate reciprocal interaction with dendritic cells, macrophages, T cells and endothelial cells and thereby affect immune response. [PMID: 18425107] Patients that experience recurrent severe herpes infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or known monogenic defects or patients who have received immune reconstitution post hematopoietic cell transplantation may lack the normal number or functionality of NK cells. The level of NK cell functionality is determined using titrations of effector cells (that are assumed to be NK cells) that are incubated with the target cells. The level of chromium released by the target cells is then divided by the corrected level of chromium measured in the positive control. The result is reported as a percent.( http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/28562)
 
 

BASIC ATTRIBUTES
  Class/Type: HEM/BC/Lab
  First Released in Version: 2.58
  Last Updated in Version: 2.58
  Order vs. Obs.: Observation
  Status: Active

MEMBER OF THESE PANELS
  82720-4   Natural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel: -: Pt: Bld MC: -:

PARTS

Part Type    Part No.  Part Name   
Component   LP230183-8  Natural killer cell cytotoxicity.12.5:1 effector to target cell ratio 
     Divisors   LP204182-2  Positive control 
Property   LP35077-4  RelACnc   [Relative Arbitrary Concentration] 
Time   LP6960-1  Pt   [Point in time (spot)] 
System   LP193850-7  Bld MC   [Blood mononuclear cells] 
Scale   LP7753-9  Qn 
Fragments for synonyms   LP89546-3  Natural killer 
Fragments for synonyms   LP21265-1 
Fragments for synonyms   LP21065-5  Positive 

RELATED NAMES
  Blood Point in time Random
  HEMATOLOGY/CELL COUNTS Pos RlACnc
  i Pos cntrl WB
  NK QNT Whole blood
  NK cytotox.12.5:1 E:T Quan  
  PBMC Quant  
  Peripheral blood mononuclear cells Quantitative  

EXAMPLE UNITS
  Unit  Source Type
  EXAMPLE UCUM UNITS 

UNITS (INTERNAL DETAILS)
  Source Type:  EXAMPLE UCUM UNITS 
  Unit: 

CHANGE HISTORY
  Change Type: ADD

INTERNAL FIELDS
  Long Common Name: Natural killer cell cytotoxicity.12.5:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname: NK cytotox.12.5:1 E:T/Pos cntrl Bld MC
  Fully Specified Name: Natural killer cell cytotoxicity.12.5:1 effector to target cell ratio/Positive control: RelACnc: Pt: Bld MC: Qn:
     
  Component Word Count: 0
  ID: 87059
  Status (Raw): ACTIVE


82727-9   Natural killer cell cytotoxicity.25:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.25:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.25:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  

NAME
  Fully-Specified Name: 
Component   Property   Time   System   Scale   Method
Natural killer cell cytotoxicity.25:1 effector to target cell ratio/Positive control  RelACnc  Pt  Bld MC  Qn 
  Long Common Name:  Natural killer cell cytotoxicity.25:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname:  NK cytotox.25:1 E:T/Pos cntrl Bld MC

TERM DEFINITION/DESCRIPTION(S)
  The natural killer (NK) cytotoxicity profile assesses the functionality of natural killer cells and is used to evaluate patients with various conditions including recurrent, severe herpes viral infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or know monogenic defects. The NK cytotoxicity profile is also used for the evaluation of immune reconstitution post hematopoietic cell transplantation and post immunomodulatory therapy. [http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+interpretive/28562] Peripheral blood mononuclear cells (MC are used as the specimen for NK cytotoxicity assays because the cytotoxicity of the MC population is predominantly due to NK cells. In the cytotoxicity assay, samples of the patient's mononuclear cells (called 'effector cells' or E cells') are incubated with labeled target cells ('T cells') in a series of predetermined ratios, for example, 100:1 E to T, 50:1 E to T, 25:1 E to T. Cytotoxicity is measured as the amount of label released when the target cell is destroyed, and the percent cytotoxicity for a given E:T ratio is the ratio of label released when all of the target cells are destroyed using a cell lysis detergent as an experimental control. Several features of the cytotoxicity profile are important, including the maximum cytotoxicity, the relative difference between the maximum and the minimum, and the change in percent cytotoxicity between sequential dilutions. Different patterns of these measures may be associated with different disease processes. Cytotoxicity may also be reported in terms of lytic units (LOINC: 34439-0), meaning the number of E cells needed to lyse a certain number of target cells.
 
 

PART DEFINITION/DESCRIPTION(S)
  Part: Natural killer cell cytotoxicity.25:1 effector to target cell ratio
  Natural killer cells are lymphocytes that identify and lyse tumor cells and cells infected with microbes. The ability of NK cells to recognize cells as foreign is dependent upon inhibitory and activating receptors on the target cells. The inhibitory receptors distinguish between normal and tumor or infected cells by the presence or absence of the major histocompatibility complex class I. The activating receptors are NKp46, NKp30, NKp44 and NKG2D. (http://emboj.embopress.org/content/23/2/255.abstract) In addition to their cytolytic function in the innate immune system, NK cells also function to regulate reciprocal interaction with dendritic cells, macrophages, T cells and endothelial cells and thereby affect immune response. [PMID: 18425107] Patients that experience recurrent severe herpes infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or known monogenic defects or patients who have received immune reconstitution post hematopoietic cell transplantation may lack the normal number or functionality of NK cells. The level of NK cell functionality is determined using titrations of effector cells (that are assumed to be NK cells) that are incubated with the target cells. The level of chromium released by the target cells is then divided by the corrected level of chromium measured in the positive control. The result is reported as a percent.( http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/28562)
 
 

BASIC ATTRIBUTES
  Class/Type: HEM/BC/Lab
  First Released in Version: 2.58
  Last Updated in Version: 2.58
  Order vs. Obs.: Observation
  Status: Active

MEMBER OF THESE PANELS
  82720-4   Natural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel: -: Pt: Bld MC: -:

PARTS

Part Type    Part No.  Part Name   
Component   LP230184-6  Natural killer cell cytotoxicity.25:1 effector to target cell ratio 
     Divisors   LP204182-2  Positive control 
Property   LP35077-4  RelACnc   [Relative Arbitrary Concentration] 
Time   LP6960-1  Pt   [Point in time (spot)] 
System   LP193850-7  Bld MC   [Blood mononuclear cells] 
Scale   LP7753-9  Qn 
Fragments for synonyms   LP89546-3  Natural killer 
Fragments for synonyms   LP21265-1 
Fragments for synonyms   LP21065-5  Positive 

RELATED NAMES
  Blood Point in time Random
  HEMATOLOGY/CELL COUNTS Pos RlACnc
  i Pos cntrl WB
  NK QNT Whole blood
  NK cytotox.25:1 E:T Quan  
  PBMC Quant  
  Peripheral blood mononuclear cells Quantitative  

EXAMPLE UNITS
  Unit  Source Type
  EXAMPLE UCUM UNITS 

UNITS (INTERNAL DETAILS)
  Source Type:  EXAMPLE UCUM UNITS 
  Unit: 

CHANGE HISTORY
  Change Type: ADD

INTERNAL FIELDS
  Long Common Name: Natural killer cell cytotoxicity.25:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname: NK cytotox.25:1 E:T/Pos cntrl Bld MC
  Fully Specified Name: Natural killer cell cytotoxicity.25:1 effector to target cell ratio/Positive control: RelACnc: Pt: Bld MC: Qn:
     
  Component Word Count: 0
  ID: 87060
  Status (Raw): ACTIVE


82728-7   Natural killer cell cytotoxicity.50:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.50:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.50:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  

NAME
  Fully-Specified Name: 
Component   Property   Time   System   Scale   Method
Natural killer cell cytotoxicity.50:1 effector to target cell ratio/Positive control  RelACnc  Pt  Bld MC  Qn 
  Long Common Name:  Natural killer cell cytotoxicity.50:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname:  NK cytotox.50:1 E:T/Pos cntrl Bld MC

TERM DEFINITION/DESCRIPTION(S)
  The natural killer (NK) cytotoxicity profile assesses the functionality of natural killer cells and is used to evaluate patients with various conditions including recurrent, severe herpes viral infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or know monogenic defects. The NK cytotoxicity profile is also used for the evaluation of immune reconstitution post hematopoietic cell transplantation and post immunomodulatory therapy. [http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+interpretive/28562] Peripheral blood mononuclear cells (MC are used as the specimen for NK cytotoxicity assays because the cytotoxicity of the MC population is predominantly due to NK cells. In the cytotoxicity assay, samples of the patient's mononuclear cells (called 'effector cells' or E cells') are incubated with labeled target cells ('T cells') in a series of predetermined ratios, for example, 100:1 E to T, 50:1 E to T, 25:1 E to T. Cytotoxicity is measured as the amount of label released when the target cell is destroyed, and the percent cytotoxicity for a given E:T ratio is the ratio of label released when all of the target cells are destroyed using a cell lysis detergent as an experimental control. Several features of the cytotoxicity profile are important, including the maximum cytotoxicity, the relative difference between the maximum and the minimum, and the change in percent cytotoxicity between sequential dilutions. Different patterns of these measures may be associated with different disease processes. Cytotoxicity may also be reported in terms of lytic units (LOINC: 34439-0), meaning the number of E cells needed to lyse a certain number of target cells.
 
 

PART DEFINITION/DESCRIPTION(S)
  Part: Natural killer cell cytotoxicity.50:1 effector to target cell ratio
  Natural killer cells are lymphocytes that identify and lyse tumor cells and cells infected with microbes. The ability of NK cells to recognize cells as foreign is dependent upon inhibitory and activating receptors on the target cells. The inhibitory receptors distinguish between normal and tumor or infected cells by the presence or absence of the major histocompatibility complex class I. The activating receptors are NKp46, NKp30, NKp44 and NKG2D. (http://emboj.embopress.org/content/23/2/255.abstract) In addition to their cytolytic function in the innate immune system, NK cells also function to regulate reciprocal interaction with dendritic cells, macrophages, T cells and endothelial cells and thereby affect immune response. [PMID: 18425107] Patients that experience recurrent severe herpes infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or known monogenic defects or patients who have received immune reconstitution post hematopoietic cell transplantation may lack the normal number or functionality of NK cells. The level of NK cell functionality is determined using titrations of effector cells (that are assumed to be NK cells) that are incubated with the target cells. The level of chromium released by the target cells is then divided by the corrected level of chromium measured in the positive control. The result is reported as a percent.( http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/28562)
 
 

BASIC ATTRIBUTES
  Class/Type: HEM/BC/Lab
  First Released in Version: 2.58
  Last Updated in Version: 2.58
  Order vs. Obs.: Observation
  Status: Active

MEMBER OF THESE PANELS
  82720-4   Natural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel: -: Pt: Bld MC: -:

PARTS

Part Type    Part No.  Part Name   
Component   LP230185-3  Natural killer cell cytotoxicity.50:1 effector to target cell ratio 
     Divisors   LP204182-2  Positive control 
Property   LP35077-4  RelACnc   [Relative Arbitrary Concentration] 
Time   LP6960-1  Pt   [Point in time (spot)] 
System   LP193850-7  Bld MC   [Blood mononuclear cells] 
Scale   LP7753-9  Qn 
Fragments for synonyms   LP89546-3  Natural killer 
Fragments for synonyms   LP21265-1 
Fragments for synonyms   LP21065-5  Positive 

RELATED NAMES
  Blood Point in time Random
  HEMATOLOGY/CELL COUNTS Pos RlACnc
  i Pos cntrl WB
  NK QNT Whole blood
  NK cytotox.50:1 E:T Quan  
  PBMC Quant  
  Peripheral blood mononuclear cells Quantitative  

EXAMPLE UNITS
  Unit  Source Type
  EXAMPLE UCUM UNITS 

UNITS (INTERNAL DETAILS)
  Source Type:  EXAMPLE UCUM UNITS 
  Unit: 

CHANGE HISTORY
  Change Type: ADD

INTERNAL FIELDS
  Long Common Name: Natural killer cell cytotoxicity.50:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname: NK cytotox.50:1 E:T/Pos cntrl Bld MC
  Fully Specified Name: Natural killer cell cytotoxicity.50:1 effector to target cell ratio/Positive control: RelACnc: Pt: Bld MC: Qn:
     
  Component Word Count: 0
  ID: 87061
  Status (Raw): ACTIVE


82729-5   Natural killer cell cytotoxicity.100:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.100:1 effector to target cell ratio/​Positive control in Blood mononuclear cellsNatural killer cell cytotoxicity.100:1 effector to target cell ratio/​Positive control: RelACnc: Pt: Bld MC: Qn:  

NAME
  Fully-Specified Name: 
Component   Property   Time   System   Scale   Method
Natural killer cell cytotoxicity.100:1 effector to target cell ratio/Positive control  RelACnc  Pt  Bld MC  Qn 
  Long Common Name:  Natural killer cell cytotoxicity.100:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname:  NK cytotox.100:1 E:T/Pos cntrl Bld MC

TERM DEFINITION/DESCRIPTION(S)
  The natural killer (NK) cytotoxicity profile assesses the functionality of natural killer cells and is used to evaluate patients with various conditions including recurrent, severe herpes viral infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or know monogenic defects. The NK cytotoxicity profile is also used for the evaluation of immune reconstitution post hematopoietic cell transplantation and post immunomodulatory therapy. [http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+interpretive/28562] Peripheral blood mononuclear cells (MC are used as the specimen for NK cytotoxicity assays because the cytotoxicity of the MC population is predominantly due to NK cells. In the cytotoxicity assay, samples of the patient's mononuclear cells (called 'effector cells' or E cells') are incubated with labeled target cells ('T cells') in a series of predetermined ratios, for example, 100:1 E to T, 50:1 E to T, 25:1 E to T. Cytotoxicity is measured as the amount of label released when the target cell is destroyed, and the percent cytotoxicity for a given E:T ratio is the ratio of label released when all of the target cells are destroyed using a cell lysis detergent as an experimental control. Several features of the cytotoxicity profile are important, including the maximum cytotoxicity, the relative difference between the maximum and the minimum, and the change in percent cytotoxicity between sequential dilutions. Different patterns of these measures may be associated with different disease processes. Cytotoxicity may also be reported in terms of lytic units (LOINC: 34439-0), meaning the number of E cells needed to lyse a certain number of target cells.
 
 

PART DEFINITION/DESCRIPTION(S)
  Part: Natural killer cell cytotoxicity.100:1 effector to target cell ratio
  Natural killer cells are lymphocytes that identify and lyse tumor cells and cells infected with microbes. The ability of NK cells to recognize cells as foreign is dependent upon inhibitory and activating receptors on the target cells. The inhibitory receptors distinguish between normal and tumor or infected cells by the presence or absence of the major histocompatibility complex class I. The activating receptors are NKp46, NKp30, NKp44 and NKG2D. (http://emboj.embopress.org/content/23/2/255.abstract) In addition to their cytolytic function in the innate immune system, NK cells also function to regulate reciprocal interaction with dendritic cells, macrophages, T cells and endothelial cells and thereby affect immune response. [PMID: 18425107] Patients that experience recurrent severe herpes infections, primary or secondary hemophagocytic lymphohistiocytosis, and suspected or known monogenic defects or patients who have received immune reconstitution post hematopoietic cell transplantation may lack the normal number or functionality of NK cells. The level of NK cell functionality is determined using titrations of effector cells (that are assumed to be NK cells) that are incubated with the target cells. The level of chromium released by the target cells is then divided by the corrected level of chromium measured in the positive control. The result is reported as a percent.( http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/28562)
 
 

BASIC ATTRIBUTES
  Class/Type: HEM/BC/Lab
  First Released in Version: 2.58
  Last Updated in Version: 2.58
  Order vs. Obs.: Observation
  Status: Active

MEMBER OF THESE PANELS
  82720-4   Natural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel - Blood mononuclear cellsNatural killer cell cytotoxicity panel: -: Pt: Bld MC: -:

PARTS

Part Type    Part No.  Part Name   
Component   LP230182-0  Natural killer cell cytotoxicity.100:1 effector to target cell ratio 
     Divisors   LP204182-2  Positive control 
Property   LP35077-4  RelACnc   [Relative Arbitrary Concentration] 
Time   LP6960-1  Pt   [Point in time (spot)] 
System   LP193850-7  Bld MC   [Blood mononuclear cells] 
Scale   LP7753-9  Qn 
Fragments for synonyms   LP89546-3  Natural killer 
Fragments for synonyms   LP21265-1 
Fragments for synonyms   LP21065-5  Positive 

RELATED NAMES
  Blood Point in time Random
  HEMATOLOGY/CELL COUNTS Pos RlACnc
  i Pos cntrl WB
  NK QNT Whole blood
  NK cytotox.100:1 E:T Quan  
  PBMC Quant  
  Peripheral blood mononuclear cells Quantitative  

EXAMPLE UNITS
  Unit  Source Type
  EXAMPLE UCUM UNITS 

UNITS (INTERNAL DETAILS)
  Source Type:  EXAMPLE UCUM UNITS 
  Unit: 

CHANGE HISTORY
  Change Type: ADD

INTERNAL FIELDS
  Long Common Name: Natural killer cell cytotoxicity.100:1 effector to target cell ratio/Positive control in Blood mononuclear cells
  Shortname: NK cytotox.100:1 E:T/Pos cntrl Bld MC
  Fully Specified Name: Natural killer cell cytotoxicity.100:1 effector to target cell ratio/Positive control: RelACnc: Pt: Bld MC: Qn:
     
  Component Word Count: 0
  ID: 87062
  Status (Raw): ACTIVE